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Walking-induced vibration control in wood floors is a critical issue attracting the attention of many researchers and engineers. This paper presents an experimental study applying static deflection tests, modal tests, and pedestrian load tests to a series of full-scale 12 m span tooth plates connected to wood truss joist floors with strongbacks and partition walls. A comparison of the calculation error of vibration parameters between the theoretical formula and a numerical model was also conducted. The results show that strongbacks and partition walls effectively reduce both the vertical displacement and the root means acceleration at the center of the floor under pedestrian load but increases the natural frequency. The partition wall can achieve a better vibration-reduction effect than strongbacks. The error of the finite element model is higher than that of the theoretical formula. Using the theoretical formula in engineering wood floor design is recommended.