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A novel acridine-based fluorescent chemosensor, <b>BK</b> ((E)-2-((acridine-9-ylimino)methyl)-N-benzhydrylhydrazine-1-carbothioamide), for monitoring ClO⁻ was prepared. The sensor <b>BK</b> was synthesized by introducing a new synthetic route of making aldehyde group using formic hydrazide. Probe <b>BK</b> displayed notable fluorescence quenching in the presence of ClO⁻ and showed a great selectivity over other guest analytes. The detection limit was calculated to be 7.65 μM. Additionally, <b>BK</b> was satisfactorily applied for sensing ClO⁻ in water samples and zebrafish.