A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
oleh: Grégory Menchon, Andrea E. Prota, Daniel Lucena-Agell, Pascal Bucher, Rolf Jansen, Herbert Irschik, Rolf Müller, Ian Paterson, J. Fernando Díaz, Karl-Heinz Altmann, Michel O. Steinmetz
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-05-01 |
Deskripsi
Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified ligands in complex with tubulin.