Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
oleh: Daniel C. Volke, Román A. Martino, Ekaterina Kozaeva, Andrea M. Smania, Pablo I. Nikel
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-05-01 |
Deskripsi
Rapid engineering of bacterial genomes is a requisite for both fundamental and applied studies. Here the authors develop an enhanced, broad-host-range cytidine base editor that enables multiplexed and efficient genome editing of Gram-negative bacteria.