Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing
oleh: Fabiola Mancini, Fabrizio Barbanti, Maria Scaturro, Giulia Errico, Angelo Iacobino, Antonino Bella, Flavia Riccardo, Giulia Marsili, Paola Stefanelli, Patrizio Pezzotti, Giovanni Rezza, Alessandra Ciervo
| Format: | Article |
|---|---|
| Diterbitkan: | Taylor & Francis Group 2020-01-01 |
Deskripsi
ABSTRACTThe RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.