Optogenetic control of protein binding using light-switchable nanobodies
oleh: Agnieszka A. Gil, César Carrasco-López, Liyuan Zhu, Evan M. Zhao, Pavithran T. Ravindran, Maxwell Z. Wilson, Alexander G. Goglia, José L. Avalos, Jared E. Toettcher
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-08-01 |
Deskripsi
The ability to regulate nanobody affinity with light would expand the applications toolbox for these reagents. Here the authors insert an optimised photoswitchable AsLOV2 domain into multiple nanobodies and demonstrate photoswitchable binding to fluorescent proteins and endogenous proteins in cells.