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The purpose of this article was to introduce the χ2 test for the data of four-fold tables collected from the cross-sectional design and the realization of SAS and R software. Specifically， three methods were introduced， namely " Pearson΄s χ2 test"， "corrected Pearson΄s χ2 test" and "likelihood ratio χ2 test". The contents involved the test hypotheses， calculation principles and formulas， prerequisites， implementation of SAS and R software， interpretation of results and statement of conclusions of the aforementioned three statistical analysis methods.