Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
oleh: Chunyu Liao, Fani Ttofali, Rebecca A. Slotkowski, Steven R. Denny, Taylor D. Cecil, Ryan T. Leenay, Albert J. Keung, Chase L. Beisel
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-07-01 |
Deskripsi
CRISPR array generation is difficult due to reoccurring repeat sequences. Here the authors present CRATES—a modular, one-pot assembly method—and demonstrate the creation of arrays for Cas9, Cas12a and Cas13a for cell-free, bacterial, yeast and mammalian systems.