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External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify <i>Candida auris</i>
oleh: Jochem B. Buil, Henrich A. L. van der Lee, Ilse Curfs-Breuker, Paul E. Verweij, Jacques F. Meis
Format: | Article |
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Diterbitkan: | MDPI AG 2019-10-01 |
Deskripsi
Background: <i>Candida auris</i> is a yeast that is causing nosocomial outbreaks in healthcare facilities around the world. There is a risk of the misidentification of <i>C. auris</i> with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)—when libraries are used that lack <i>C. auris</i> spectra, or when conventional biochemical methods are used. Methods: We conducted an external quality assessment to evaluate the ability of Dutch clinical microbiological laboratories to identify <i>C. auris</i>, and to raise awareness about the risk of misidentification. Results: 35/47 participating laboratories were able to identify <i>C. auris</i> correctly. Only 2/14 labs that potentially misidentified <i>C. auris</i> with their primary identification methods specified that they would perform additional tests to exclude <i>C. auris</i> when appropriate. 45/47 labs used MALDI-TOF MS systems to identify <i>Candida</i> species. Conclusions: There was a lack of awareness about the potential misidentification of <i>C. auris</i> in many labs that used MALDI-TOF MS with libraries that lacked <i>C. auris</i> spectra, and labs that used Vitek 2. However, as the currently available MALDI-TOF MS libraries in The Netherlands contain several <i>C. auris</i> spectra, we expect that currently almost all participating laboratories are able to identify <i>C. auris</i> correctly, as 45/47 participating laboratories use MALDI-TOF MS as their primary yeast identification method.