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In recent years, with the improvement of large-scale planting in rural areas, greenhouse cultivation technology has been developed rapidly. Intelligent monitoring and control of modern agricultural greenhouse is a hot topic at present. Based on the design requirements of modern greenhouse and the architecture of software design, this paper designs and develops the main program, data acquisition subroutine, alarm subroutine, control keystroke subroutine and display driver subroutine of the system based on the STM32F103ZET6 main control chip, and realizes the design of intelligent monitoring and control system of greenhouse, This study is of great significance for the establishment of scientific ecological environment of plants and the realization of high quality and high yield of crops.