Seqüenciamento de peptídeos usando espectrometria de massas: um guia prático Peptide sequencing using mass spectrometry: a practical guide
oleh: Marcelo Delmar Cantú, Emanuel Carrilho, Nelson Arno Wulff, Mario Sérgio Palma
| Format: | Article |
|---|---|
| Diterbitkan: | Sociedade Brasileira de Química 2008-01-01 |
Deskripsi
<abstract language="eng">This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.