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Antibiotic resistance in fungal isolates is increasing on a global scale. Despite knowledge that pig farmers are occupationally exposed to infectious species of fungi, such as <i>Aspergillus</i> spp., little is known regarding their potential exposure to antifungal-resistant <i>Aspergillus</i> spp. The aim of this study is to obtain knowledge regarding the antifungal resistance profiles of isolates of <i>Aspergillus</i> species taken from different source materials—including airborne dust, surface dust, faeces, and straw—within a pig farm. The EUCAST broth microdilution method was used for testing antifungal resistance from 43 isolates of <i>Aspergillus</i> sampled in 3 periods inside a Danish pig farm. Seven species of <i>Aspergillus</i> were obtained, including <i>A. candidus</i> (<i>n</i> = 5)<i>, A. fumigatus</i> (<i>n</i> = 5)<i>, A. glaucus</i> (<i>n</i> = 13)<i>, A. nidulans</i> (<i>n</i> = 2)<i>, A. niger</i> (<i>n</i> = 15)<i>, A. terreus</i> (<i>n</i> = 1)<i>,</i> and <i>A. versicolor</i> (<i>n</i> = 2). Overall, 27.9% of the <i>Aspergillus</i> isolates displayed resistance against at least one antifungal, and 11.6% of <i>Aspergillus</i> isolates displayed resistance against multiple antifungals. The most abundant group exhibiting antifungal resistance was affiliated with the species <i>A. niger</i>, with isolates exhibiting resistance to itraconazole, voriconazole, and caspofungin. One isolate of <i>A. glaucus</i> and two isolates of <i>A. versicolor</i> were resistant to amphotericin B (MIC ≥ 2 mg/L amphotericin B). Antibiotic-resistant fungi were found on all three sampling days.