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The <i>MYB</i> gene family is significant in plants, playing a role in numerous plant development processes, including metabolism, hormone signal transduction, cell identity, and biotic and abiotic stresses. Due to the recent availability of the <i>Melastoma candidum</i> genome, this is the first time that the <i>MYB</i> gene family has been identified in this species. This study identified 421 <i>MYB</i> gene members in the <i>M. candidum</i> genome using the HMMER search and BLASTp method. These <i>MYBs</i> were further divided into 10 sub-types, including R2R3, R-R, CPC-like, CCA1-like, TBP-like, R1R2R3, I-box, atypical MYB, MYB-CC, and MYB-like. Domain and conservation analyses revealed that each type of MYB was characterized by a different number and combination of SANTs/myb DNA-binding domains. Collinearity analysis revealed several gene duplication events within the <i>MYB</i> gene family. The Ka to Ks ratio suggested that most of the <i>MYB</i> genes underwent purifying selection during the evolution process. Phylogenetic analysis among three species confirmed our findings and displayed the evolutionary relationship of <i>MYB</i> genes in different species. RNA-seq of three developmental stages of flowers and WGCNA analysis identified <i>McMYB113h</i>, <i>McMYB21b</i>, and <i>McGLK1c</i> as playing a pivotal role during flower development in <i>M. candidum</i>. Finally, we conducted qRT-PCR experiments for 20 flower-development-related <i>MYBs</i> across 9 tissues to illustrate their expression patterns in <i>M. candidum</i>. This study establishes a foundation for exploring <i>MYB</i> gene resources and their potential applications in related industries of <i>M. candidum</i>.