Development of primers for sequencing the NSP1, NSP3, and VP6 genes of the group A porcine rotavirus

oleh: Fernanda Dornelas Florentino Silva, Paloma Oliveira Tonietti, Luis Ramiro Luna Espinoza, Paulo Eduardo Brandão, Leonardo José Richtzenhain, Fabio Gregori

Format: Article
Diterbitkan: Universidade Estadual de Londrina 2014-02-01

Deskripsi

Rotavirus is the causative pathogen of diarrhea in humans and in several animal species. Eight pairs of primers were developed and used for Sanger sequencing of the coding region of the NSP1, NSP3, and VP6 genes based on the conserved regions of the genome of the group A porcine rotavirus. Three samples previously screened as positive for group A rotaviruses were subjected to gene amplification and sequencing to characterize the pathogen. The information generated from this study is crucial for the understanding of the epidemiology of the disease.