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This study aimed to assess the anterior corneal wavefront aberrations, keratometry, astigmatism vectors and pupil size between Pentacam HR^® (Oculus Optikgeraete GmbH, Wetzlar, Germany) and iTrace^® (Tracey Technologies Corp., Houston, TX, USA). In this observational study, 100 eyes (50 healthy volunteers) were scanned in mesopic light condition with a Pentacam HR^® and iTrace^®. Anterior corneal aberrations (spherical aberration (Z40), vertical coma (Z3 − 1), horizontal coma (Z3 + 1)), keratometry in the flattest (K1) and steepest meridian (K2), mean astigmatism, astigmatic vectors (J0 and J45), and pupil size were measured. We found a significant difference in Z40 (Pentacam^®: +0.30 ± 0.11 µm and iTrace^®: −0.03 µm ± 0.05 µm; <i>p</i> < 0.01) with no correlation between the devices (r = −0.12, <i>p</i> = 0.22). The devices were in complete agreement for Z3 − 1 (<i>p</i> = 0.78) and Z3 + 1 (<i>p</i> = 0.39), with significant correlation between the machines (r = −0.38, <i>p</i> < 0.01 and r = −0.6, <i>p</i> < 0.01). There was no difference in K1, K2 and mean astigmatism. J0 was negative with both devices (against-the-rule astigmatism), but there was no correlation. J45 was negative with the Pentacam HR^® (more myopic oblique astigmatism) but significantly correlated between the devices. Pupil size was smaller with Pentacam HR^® (<i>p</i> < 0.01). In summary, these devices cannot be used interchangeably. Corneal Z40 was significantly different with more negative Z40 with iTrace^® compared to Pentacam HR^®. iTrace^® operates with lower illumination, giving larger pupil size than Pentacam HR^®, which uses intense blue light during measurement. No correlation was found for J0. Pentacam HR^® had a trend to record more negative J45 (myopic oblique astigmatism).