Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
oleh: Arturo Carabias, Anders Fuglsang, Piero Temperini, Tillmann Pape, Nicholas Sofos, Stefano Stella, Simon Erlendsson, Guillermo Montoya
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-07-01 |
Deskripsi
The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and discuss Cas12j3 PAM recognition, hybrid stabilisation and the activation mechanism.