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The impact of changes in agricultural land use and practices as a controlling driver of hydrologic response and as a source of diffuse pollution, are studied in the Joumine River basin, discharging into the Ichkeul Lake, northern Tunisia, a UNESCO World Heritage site since 1979. The lake is characterized by a very specific hydrological functioning based on a seasonal alternation of water levels and salinity through its link to the Mediterranean Sea. Three Landsat images, <i>in situ</i> surveys and SWAT modelling were used to simulate and assess streamflows and nitrate loads under retrospective land uses.