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We tested the laboratory susceptibility of four endemic and two exotic species of North American Coccinellidae to a single rate (2.5 × 105 conidia/ml) of Beauveria bassiana derived from different sources. The endemic species Olla v-nigrum, Cycloneda munda and Hippodamia convergens were susceptible to B. bassiana Isolate B which was originally isolated from O. v-nigrum and the rate assayed was known to represent the LC50 against O. v-nigrum. However, neither the endemic C. maculata nor the exotic species Harmonia axyridis and Coccinella septempunctata were susceptible to this isolate at this rate. Additionally, all species of endemic and exotic Coccinellidae used in these assays were resistant to B. bassiana strain GHA and BbAR1 at that rate. We discuss the implications of these results in regard to the establishment of exotic species in new habitats.