Find in Library

It is a unique feature that fat can be deposited in sheep tails and rumps. To elucidate the genetic mechanism underlying this trait, we collected 120 individuals from three Chinese indigenous sheep breeds with extreme tail types, namely large fat-tailed sheep (<i>n</i> = 40), Altay sheep (<i>n</i> = 40), and Tibetan sheep (<i>n</i> = 40), and genotyped them using the Ovine Infinium HD SNP BeadChip. Then genomic scan for selection signatures was performed using the hapFLK. In total, we identified 25 genomic regions exhibiting evidence of having been under selection. Bioinformatic analysis of the genomic regions showed that selection signatures related to multiple candidate genes had a demonstrated role in phenotypic variation. Nine genes have documented association with sheep tail types, including <i>WDR92</i>, <i>TBX12</i>, <i>WARS2</i>, <i>BMP2</i>, <i>VEGFA</i>, <i>PDGFD</i>, <i>HOXA10</i>, <i>ALX4</i>, and <i>ETAA1</i>. Moreover, a number of genes were of particular interest, including <i>RXFP2</i> associated with the presence/absence and morphology of horns; <i>MITF</i> involved in coat color; <i>LIN52</i> and <i>SYNDIG1L</i> related to the number of teats; <i>MSRB3</i> gene associated with ear sizes; <i>LTBP2</i> considered as a positional candidate genes for number of ribs; <i>JAZF1</i> regulating lipid metabolism; <i>PGRMC2</i>, <i>SPAG17</i>, <i>TSHR</i>, <i>GTF2A1</i>, and <i>LARP1B</i> implicated with reproductive traits. Our findings provide insights into fat tail formation and a reference for carrying out molecular breeding and conservation in sheep.