Simultaneous expression of displayed and secreted antibodies for antibody screen.
oleh: Yuanping Zhou, Junjie Wang, Ivan Zhou, Haibo Lou, Chang-Zheng Li, Zhen-Rui Chen, Zhe-Huan Zhang, Shuwen Liu, Shuguang Wu, Wanlong Tan, Shibo Jiang, Chen Zhou
| Format: | Article |
|---|---|
| Diterbitkan: | Public Library of Science (PLoS) 2013-01-01 |
Deskripsi
The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmembrane domain to obtain secreted antibodies. As a result, antibodies can be expressed simultaneously on the cell surface in a membrane-anchored version for screening and selecting through fluorescence-activated cell sorting (FACS) analysis, as well as in conditioned medium in a secreted version for function analysis.