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Renewable biomass as a sustainable feedstock has been enormously explored for the manufacturing of high value-added products such as biofuels, commodity chemicals, and new bio-based materials. The present paper describes the synthesis of a series of natural zeolite based catalysts by loading different weight percentage of phosphomolybdic acid. The efficiency of catalysts were checked by conversion of carbohydrate (glucose and fructose) to 5-HMF, which is an important industrial chemical used in the production of various value-added chemicals, materials and biofuels.