Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
oleh: Marcos César Lima de Mendonça, Ana Maria de Amorim Ferreira, Marta Gonçalves Matos dos Santos, Elva Cristina Oviedo, Maria Sônia Dal Bello, Marilda Mendonça Siqueira, Juan Manuel Piñeiro Maceira, Maria Genoveva von Hubinger, José Nelson dos Santos Silva Couceiro
| Format: | Article |
|---|---|
| Diterbitkan: | Fundação Oswaldo Cruz (FIOCRUZ) 2011-06-01 |
Deskripsi
Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.