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This in vitro study evaluated the surface hydrophobicity of 3D-printed denture base resin exposed to either an extended post-curing light exposure time or a sandblasting procedure. MATERIALS AND METHODS: Disk-shaped samples (diameter × height: 10 mm × 3 mm) were 3D-printed with stereolithography SLA technology using the denture-base resin. Samples were divided into three groups: control, extended UV-post-curing, and sandblasted. The surface roughness parameters for each group were calculated, and the surface hydrophobicity was evaluated by measuring the drop contact angle. Analysis was done using the T-test; significance was when <i>p</i> < 0.05. RESULTS: The comparison of surface roughness parameters showed significant differences between the control group and the sandblasted group (Sa: <i>p</i> = 0.001, Sz: <i>p</i> < 0.001, Str: <i>p</i> < 0.001, Spc: <i>p</i> = 0.044) as well as between the extended-cure group and the sandblasted group (Sa: <i>p</i> = 0.006, Sz: <i>p</i> < 0.001, Str: <i>p</i> < 0.001, Spc: <i>p</i> = 0.036) except for the Sdr measures. The surface hydrophobicity was also statistically lower in the sandblasted group compared to both the control and extended curing groups (<i>p</i> < 0.001). CONCLUSION: The sandblasting procedure created a less hydrophobic surface of the 3D-printed denture base resin, and the altered surface roughness could be a contributor to this observation.