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In this study, first appearance time of the alpha-naphthyl acetate esterase positive lymphocytes in the fetal peripheral blood were determined by demonstrating of the alpha-naphthyl acetate esterase. Positively stained lymphocytes were first seen in the peripheral blood smears of the fetuses in 60 days old.<p> Large majority of the alpha-naphthyl acetate esterase pozitive lymphocytes showed localised granularpositivity, whereas, in the smaller numbers of positive cells, a diffuse granular staining pattern was observed.<p> Neutrophyls were negative. Monocytes displayed a diffuse granular staining pattern.<p> The frequency of the alpha-naphthyl acetate esterase positive lymphocytes increased with the fetal age and the highest levels were determined in the 240 th day of the gestation.