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The aim of this study was to analyse the effect of fat replacement by oat β-glucan concentrates on the physicochemical properties of beef burgers. The goal was to obtain a low-fat product accepted by consumers, having physicochemical parameters similar to the control sample, composed of β-glucan at least by 1%. This evaluation was based on basic composition, β-glucan and cholesterol content, colour and texture analysis, pH value, WHC, volatile compounds profile and sensory properties of burgers. Burgers with β-glucan concentrates had lower cholesterol content and higher WHC and texture (hardness, cohesiveness and springiness increase) parameters than control burgers. The highest overall acceptance scores among the samples with β-glucan were recorded for the sample with β-glucan 30% concentrate in amount of 4%. This modification could allow the substitution of traditional beef burgers with a healthier low-fat meat product with similar customer appeal.