Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9
oleh: Hyang-Hee Seo, Hyo-Won Han, Jung-Hyun Kim
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2020-05-01 |
Deskripsi
Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities.