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In the present study, thirty two lactic acid bacteria (LAB) were isolated from fermented Indian herbal medicine. In comparison to other strains, MNL5 had stronger bile salt hydrolase (BSH) and cholesterol-lowering properties. Furthermore, it can withstand the extreme conditions found in the GI tract, due to, e.g., pepsin, bile salts, pancreatin, and acids. <i>Pediococcus acidilactici</i> MNL5 was identified as a probiotic candidate after sequencing the 16S rRNA gene. The antibacterial activity of <i>P. acidilactici</i> MNL5 cell-free supernatants (CFS) against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Helicobacter pylori, Bacillus cereus</i>, and <i>Candida albicans</i> was moderate. A <i>Caenorhabditis elegans</i> experiment was also performed to assess the effectiveness of <i>P. acidilactici</i> MNL5 supplementation to increase life span compared to <i>E. coli</i> supplementation (DAF-2 and LIU1 models) (<i>p</i> < 0.05). An immense reduction of the lipid droplets of <i>C. elegans</i> was identified through a fluorescent microscope. The drastic alteration of the expression of fat genes is related to obesity phenotypes. Hence, several paths are evolutionary for <i>C. elegans</i>; the results of our work highlight the nematode as an important model for obesity.