Phage-assisted continuous evolution of proteases with altered substrate specificity
oleh: Michael S. Packer, Holly A. Rees, David R. Liu
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-10-01 |
Deskripsi
Proteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence specificities.