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Physiome is an area of physiology to generate a whole system by analyzing and integrating scattered and discrete information. The term “physiome” was first introduced by James B. Bassingthwaight in 1993, and officially announced by the International Union of Physiological Societies as the new field to be accomplished in the 21st century. In this review, I introduce the concepts of physiome, why physiome should be pursued, what kind of strategy is necessary to form physiome, and how physiome can be used.