The generation and use of recombinant extracellular vesicles as biological reference material
oleh: Edward Geeurickx, Joeri Tulkens, Bert Dhondt, Jan Van Deun, Lien Lippens, Glenn Vergauwen, Elisa Heyrman, Delphine De Sutter, Kris Gevaert, Francis Impens, Ilkka Miinalainen, Pieter-Jan Van Bockstal, Thomas De Beer, Marca H. M. Wauben, Esther N. M. Nolte-‘t-Hoen, Katarzyna Bloch, Johannes V. Swinnen, Edwin van der Pol, Rienk Nieuwland, Geert Braems, Nico Callewaert, Pieter Mestdagh, Jo Vandesompele, Hannelore Denys, Sven Eyckerman, Olivier De Wever, An Hendrix
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-07-01 |
Deskripsi
There is no universal reference material to develop extracellular vesicle (EV) separation methods and carry out calibration and normalization. Here the authors use HIV-derived gag proteins to assemble recombinant fluorescent EV as a trackable reference material resembling the physical and biochemical properties of sample EV.