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We have studied the possible isospin corrections on the skewness and kurtosis of net-baryon and net-charge fluctuations in the isospin asymmetric matter formed in relativistic heavy-ion collisions at RHIC-BES energies, based on a 3-flavor Polyakov-looped Nambu–Jona–Lasinio model. With typical scalar–isovector and vector–isovector couplings leading to the splitting of <i>u</i> and <i>d</i> quark chiral phase transition boundaries and critical points, we have observed considerable isospin effects on the susceptibilities, especially those of net-charge fluctuations. Reliable experimental measurements at even lower collision energies are encouraged to confirm the observed isospin effects.