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Five Vitis vinifera L. cultivars Shiraz, Cabernet Sauvignon, Riesling, Chardonnay and Pinot Gris at different E-L development stages were harvested in two experimental vintages. Temperature and rainfall data of the growing period were obtained from the Australian Government Bureau of Meteorology. Free terpene concentrations of all harvested grape samples were analysed using HS-SPME-GC-MS. One-way ANNOVA was performed to evaluate the significance of changes in terpene concentrations at different maturation stages. More analysis of the data is provided in “Free terpene evolution during the berry maturation of five Vitis vinifera L. cultivars” [1]. Keywords: Shiraz, Cabernet Sauvignon, Riesling, Chardonnay, Pinot Gris, terpene, Evolution, SPME-GC-MS