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Reversible phosphorylation of proteins is a ubiquitous regulatory mechanism in vivo that can respond to external changes, and plays an extremely important role in cell signal transduction. Protein phosphatase 2C is the largest protein phosphatase family in higher plants. Recently, it has been found that some clade A members can negatively regulate ABA signaling pathways. However, the functions of several subgroups of <i>Arabidopsis</i> PP2C other than clade A have not been reported, and whether other members of the PP2C family also participate in the regulation of ABA signaling pathways remains to be studied. In this study, based on the previous screening and identification work of PP2C involved in the ABA pathway, the clade F member PIA1 encoding a gene of the <i>PP2C</i> family, which was down-regulated after ABA treatment during the screening, was selected as the target. Overexpression of <i>PIA1</i> significantly down-regulated the expression of ABA marker gene <i>RD29A</i> in <i>Arabidopsis</i> protoplasts, and ABA-responsive elements have been found in the cis-regulatory elements of <i>PIA1</i> by promoter analysis. When compared to Col-0, transgenic plants overexpressing <i>PIA1</i> were less sensitive to ABA, whereas <i>pia1</i> showed the opposite trait in seed germination, root growth, and stomatal opening experiments. Under drought stress, SOD, POD, CAT, and APX activities of <i>PIA1</i> overexpression lines were lower than Col-0 and <i>pia1</i>, while the content of H₂O₂ was higher, leading to its lowest survival rate in test plants, which were consistent with the significant inhibition of the expression of ABA-dependent stress-responsive genes <i>RD29B</i>, <i>ABI5</i>, <i>ABF3</i>, and <i>ABF4</i> in the <i>PIA1</i> transgenic background after ABA treatment. Using yeast two-hybrid and luciferase complementation assays, PIA1 was found to interact with multiple ABA key signaling elements, including 2 RCARs and 6 SnRK2s. Our results indicate that <i>PIA1</i> may reduce plant drought tolerance by functioning as a common negative regulator involved in ABA signaling pathway.