A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

oleh: Mikhail E. Matlashov, Daria M. Shcherbakova, Jonatan Alvelid, Mikhail Baloban, Francesca Pennacchietti, Anton A. Shemetov, Ilaria Testa, Vladislav V. Verkhusha

Format: Article
Diterbitkan: Nature Portfolio 2020-01-01

Deskripsi

Monomeric near-infrared (NIR) fluorescent proteins (FPs) from bacterial phytochromes bring potential advantages, but their brightness in cells is lower than dimeric NIR FPs. Here the authors develop enhanced monomeric NIR FPs enabling imaging across different scales without the trade-off between brightness and monomeric state.