Co-Localization of Resistance and Metabolic Quantitative Trait Loci on Carrot Genome Reveals Fungitoxic Terpenes and Related Candidate Genes Associated with the Resistance to <i>Alternaria dauci</i>
oleh: Claude Emmanuel Koutouan, Valérie Le Clerc, Anita Suel, Latifa Hamama, Patricia Claudel, David Halter, Raymonde Baltenweck, Philippe Hugueney, Jean-François Chich, Sitti Anlati Moussa, Clémentine Champlain, Sébastien Huet, Linda Voisine, Sandra Pelletier, Sandrine Balzergue, Wilfried Chevalier, Emmanuel Geoffriau, Mathilde Briard
| Format: | Article |
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| Diterbitkan: | MDPI AG 2023-01-01 |
Deskripsi
Alternaria leaf blight, caused by the fungus <i>Alternaria dauci</i>, is the most damaging foliar disease of carrot. Some carrot genotypes exhibit partial resistance to this pathogen and resistance Quantitative Trait Loci (rQTL) have been identified. Co-localization of metabolic QTL and rQTL identified camphene, α-pinene, α-bisabolene, β-cubebene, caryophyllene, germacrene D and α-humulene as terpenes potentially involved in carrot resistance against ALB. By combining genomic and transcriptomic analyses, we identified, under the co-localization regions, terpene-related genes which are differentially expressed between a resistant and a susceptible carrot genotype. These genes include five terpene synthases and twenty transcription factors. In addition, significant mycelial growth inhibition was observed in the presence of α-humulene and caryophyllene.