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<strong>Introduction</strong>: Consumption of appropriate amount of probiotic microorganisms via food products have health benefits on the host. In recent years, there has been a significant increase in research on the characterization and verification potential use of probiotic films in food industry. The aim of the current study was to investigate <em>in vitro</em> antimicrobial property of probiotic carboxymethyl cellulose-sodium caseinate (CMC-SC) films containing <em>Lactobacillus acidophilus</em>, <em>L. reuteri</em> and <em>Bifidobacterium bifidum </em>against <em>Listeria monocytogenes</em>, <em>Salmonella typhimurium</em>, <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> O157:H7. <strong>Methods</strong>: Preparation of CMC-SC composite films were conducted based on casting method. The <em>in vitro</em> antibacterial property of CMC-SC films was evaluated using agar disk diffusion and broth micro-dilution methods. <strong>Results</strong>: Antimicrobial property of probiotic films (diameter inhibition zone and log differences in population, respectively) were as follow: <em>S. aureus</em> (2.13-5.65 mm and -0.79 - -3.82) > <em>L. monocytogenes </em>(1.76-5.32 mm and -0.65 - -3.34) > <em>S. typhimurium</em> (2.13-4.33 mm and -0.34 - -2.79) > <em>E. coli</em> O157:H7 (1.88-3.86 mm and -0.18 - -2.62). The best antimicrobial property against aforementioned bacterial pathogens was found for film supplemented with <em>L. acidophilus </em>+<em> L. reuteri </em>+ <em>B. bifidum. </em><strong>Conclusion</strong>: It can be concluded that incorporation of some probiotic strains into edible films resulted in excellent antimicrobial property against <em>S. aureus</em>, <em>L. monocytogenes, S. typhimurium</em> and <em>E. coli</em> O157:H7 and probably solve safety related issue in food industry.