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The genetic diversity of 37 genotypes of Chrysanthemum was characterized using inter simple sequence repeats (ISSR) technique. A total of 46 primers were screened, of which 10 polymorphic and informative patterns were selected to determine genetic relationships. Among 114 amplified DNA fragments obtained, 107 bands were polymorphic, accounting for 93.86% polymorphism. The percentage of polymorphism exhibited by different ISSR primers ranged from 71.43% (ISSR-825) to 100% (ISSR-808, 810, 812, 840 and 842). The similarity coefficient values ranged from 0.275 to 0.775 with a mean similarity matrix of 0.525. Among the 37 genotypes studied, the closest relationship was scored between Geetanjali and Red Stone with similarity level of 77.5% while, the most distantly related genotypes were Autumn Joy and Flirtation with the lowest similarity index of 0.275. The UPGMA dendrogram revealed that the chrysanthemum genotypes were grouped into ten clusters.