Generation of an induced pluripotent stem cell line, FJMUUHi001-A, from a hereditary Parkinson's disease patient with homozygous mutation of c.189dupA in PARK7
oleh: Zhi-ting Chen, Zhen-hua Zhao, Li-na Chen, Fei Fan, Guo-en Cai, Hui-dan Weng, Ying-qing Wang, Lian-ming Liao, Xiao-chun Chen, En Huang, Qin-yong Ye
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-03-01 |
Deskripsi
PARK7 mutations are accountable for the inherited Parkinson's disease. An induced pluripotent stem cell (iPSC) line FJMUUHi001-A was generated by expressing five reprogramming factors, OCT3/4, SOX2, c-MYC, KLF4 and BCL-XL, in peripheral blood mononuclear cells from a 32-year old patient carrying a homozygous mutation of c.189dupA in PARK7. The iPSCs with a normal karyotype had the abilities to differentiate into three germ layers and expressed pluripotency markers without detectable residual plasmids. The cell line FJMUUHi001-A carrying the truncating protein PARK7 could be a useful tool to help comprehend the function of PARK7 in the iPSCs and differentiated cells from them.