Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences
oleh: Amanda R. Krysler, Christopher R. Cromwell, Tommy Tu, Juan Jovel, Basil P. Hubbard
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-03-01 |
Deskripsi
Genetic variance poses a major challenge for CRISPR-based human therapeutics and pathogen diagnostics. Here the authors show how to circumvent this issue by using guide RNAs containing universal bases to tailor Cas9/Cas12 specificity.