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Although the plants of the genus <i>Euphorbia</i> are largely exploited by therapists in Morocco, the composition and antibacterial activities of propolis from these plants are still unknown. To address this gap, this study aimed to characterize the pollen type, the volatile compounds, and the phenolic and mineral profiles of three <i>Euphorbia</i> propolis samples collected in Morocco and evaluate their antimicrobial activities. The minimum inhibitory concentration of the propolis samples was determined by the microdilution method, and the anti-adherence activity was evaluated by the crystal violet assay. The examination of anti-quorum-sensing proprieties was performed using the biosensor <i>Chromobacterium violaceum</i> CV026. Pollen analysis revealed that <i>Euphorbia resinifera</i> pollen dominated in the P1 sample (58%), while <i>E. officinarum</i> pollen dominated in the P2 and P3 samples (44%). The volatile compounds were primarily composed of monoterpene hydrocarbons, constituting 35% in P1 and 31% in P2, with α-pinene being the major component in both cases, at 16% in P1 and 15% in P2. Calcium (Ca) was the predominant mineral element in both <i>E. resinifera</i> (P1) and <i>E. officinarum</i> (P2 and P3) propolis samples. Higher levels of phenols, flavonoids and dihydroflavonoids were detected in the <i>E. officinarum</i> P2 sample. The minimum inhibitory concentration (MIC) value ranged from 50 to 450 µL/mL against Gram-positive and Gram-negative bacteria. <i>Euphorbia</i> propolis displayed the ability to inhibit quorum sensing in the biosensor <i>C. violaceum</i> CV026 and disrupted bacterial biofilm formation, including that of resistant bacterial pathogens. In summary, the current study evidences the potential use of <i>E. officinarum</i> propolis (P2 and P3) to combat important features of resistant pathogenic bacteria, such as quorum sensing and biofilm formation.