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The complete mitochondrial genome (mitogenome) of Hydrellia griseola has been reported in this study. This is the first sequenced complete mitogenome of the family Ephydridae. The complete mitogenome is 16,159 bp in length, including 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and a partial sequence of the AT-rich region, and the AT-rich region contains several characteristic repeated sequences. In addition, the nucleotide composition of the coding region was 38.7% of A, 37.0% of T, 14.2% of C, 10.1% of G, 75.7% of A + T content. Four complete mitochondrial genome data of related species are download from GenBank, and all of them are used in Neighbor-Join analyses. The result consistently supports the monophyly of Ephydroidea.