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<b>Objective:</b> To established a method for determination of 21 perfluorinated compounds in animal-derived food by ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. <b>Methods:</b> The sample was extracted with 1% formic acid-acetonitrile, cleaned up with a sorbent mixture of <i>N</i>-propylethylenediamine （PSA）, octadecyl bonded silica gel （C₁₈） and graphitized carbon black （GCB）, then separated by acetonitrile-5mmol/L ammonium acetate aqueous solution and Acquity UPLC^® BEH C₁₈, detected by liquid chromatography tandem mass spectrometry in negative mode using the multiple reaction monitoring （MRM） mode. <b>Results:</b> The calibration curves were linear in the range of 0.2~10 ng/mL for the 21 PFAS. The limits of quantification were 0.25 μg/kg. Average recoveries were in the range of 71%~118% with three spiked levels, namely 0.25, 0.50, 1.25 μg/kg. The relative standard deviations （RSDs%） of inter-batch and intra-batch were 3.5%~18.3% （<i>n</i>=6） and 2.2%~13.8% （<i>n</i>=3）, respectively. <b>Conclusion:</b> The simple pretreatment and high sensitivity satisfied the fast and accurate detection requirement, meanwhile it is suitable for qualitative and quantitative analysis of perfluorinated compounds in animal-derived food.