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Tubercular involvement of central nervous system is one of the common extrapulmonary manifestations of tuberculosis. MRI brain plays an important role in diagnosis of CNS tuberculomas. Most of the articles on MR spectroscopy of CNS tuberculomas have mentioned presence of lipid peak within it. However, few recent studies have mentioned presence of singlet peak at 3.8 ppm corresponding to Guanidinoacetate as more specific finding in CNS tuberculoma. Here we present Magnetic Resonance spectroscopy MRS findings of singlet peak at 3.8 ppm in three confirmed cases of CNS tuberculomas with review of literature.