Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy
oleh: Murat Yildirim, Hiroki Sugihara, Peter T. C. So, Mriganka Sur
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-01-01 |
Deskripsi
Two-photon microscopy is a powerful tool for studying neuronal activity but cannot easily image deeper cortical layers. Here, the authors design a custom microscope for three-photon microscopy and use it to reveal response properties of layer 5, 6, and subplate visual cortical neurons.