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The present study was designed to compare the photometer (Lp 300 SDM Minitüb GmHb) with improved Neubauer hemocytometer and Makler counting chamber for sperm concentration measurement in cow bulls. Data were based on 35 cow bull semen samples. The average sperm concentrations (109/ml) determined by photometer, hemocytometer and Makler chamber were 1.35±0.72, 1.17±0.53 and 1.49±0.60, respectively. Analysis of variance revealed that there was no difference among the three techniques of sperm concentration measurement of same semen samples in cow bulls. It was concluded that the use of photometer in semen evaluation for sperm concentration reduced chances of human error and time consumption effectively.