Introducing the Newly Isolated Bacterium <i>Aneurinibacillus</i> sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium
oleh: Iva Pernicova, Ivana Novackova, Petr Sedlacek, Xenie Kourilova, Michal Kalina, Adriana Kovalcik, Martin Koller, Jana Nebesarova, Vladislav Krzyzanek, Kamila Hrubanova, Jiri Masilko, Eva Slaninova, Stanislav Obruca
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2020-05-01 |
Deskripsi
Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolate <i>Aneurinibacillus</i> sp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 °C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated that <i>Aneurinibacillus</i> sp. H1 is a very promising candidate for production of PHA with tailored material properties.