BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

oleh: Michael Zabolocki, Kasandra McCormack, Mark van den Hurk, Bridget Milky, Andrew P. Shoubridge, Robert Adams, Jenne Tran, Anita Mahadevan-Jansen, Philipp Reineck, Jacob Thomas, Mark R. Hutchinson, Carmen K. H. Mak, Adam AƱonuevo, Leon H. Chew, Adam J. Hirst, Vivian M. Lee, Erin Knock, Cedric Bardy

Format: Article
Diterbitkan: Nature Portfolio 2020-11-01

Deskripsi

Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.