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Chromium in wastewater is classified as one of dangerous contaminants that requires further treatment before it can be discharged to water body. Concentration of chromium in water body, especially river, has increased as many industries are utilizing chromium as raw materials and then discharging its wastewater directly without any treatment. Biosorption is one of methods that widely used to treat heavy metal containing wastewater. Bacteria is the most common microorganism to be used as heavy metal treatment agent. Azotobacter s8, Bacillus subtilis and Pseudomonas putida had been proven to have a heavy metal resistant capability. Screening test showed that Minimum Inhibitory Concentration value of chromium for all bacteria were ranged from 100 to 250 mg/L of CrCl3. Chromium biosorption test by bacteria showed that Azotobacter s8 was able to remove 10.53%, and Bacillus subtilis was able to remove 5.68% chromium from 50 mg/L initial concentration while Pseudomonas putida showed no chromium removal. The chromium biosorption capacity by Azotobacter s8 was 580.08 mg/g and 349.30 mg/g for Bacillus subtilis.