Full-Length Transcriptome Sequencing Provides Insights into Flavonoid Biosynthesis in <i>Fritillaria hupehensis</i>

oleh: Kunyuan Guo, Jie Chen, Yan Niu, Xianming Lin

Format: Article
Diterbitkan: MDPI AG 2021-03-01

Deskripsi

One of the most commonly utilized medicinal plants in China is <i>Fritillaria hupehensis</i> (Hsiao et K.C. Hsia). However, due to a lack of genomic resources, little is known about the biosynthesis of relevant compounds, particularly the flavonoid biosynthesis pathway. A PacBio RS II sequencing generated a total of 342,044 reads from the bulb, leaf, root, and stem, of which 316,438 were full-length (FL) non-redundant reads with an average length of 1365 bp and a N50 of 1888 bp. There were also 38,607 long non-coding RNAs and 7914 simple sequence repeats detected. To improve our understanding of processes implicated in regulating secondary metabolite biosynthesis in <i>F. hupehensis</i> tissues, we evaluated potential metabolic pathways. Overall, this study provides a repertoire of FL transcripts in <i>F. hupehensis</i> for the first time, and it will be a valuable resource for marker-assisted breeding and research into bioactive compounds for medicinal and pharmacological applications.