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Heat shock transcription factors (HSFs) are among the most important regulators of plant responses to abiotic stimuli. They play a key role in numerous transcriptional regulatory processes. However, the specific characteristics of HSF gene family members and their expression patterns in different tissues and under drought stress have not been precisely investigated in <i>Heimia myrtifolia</i>. This study analyzed transcriptome data from <i>H. myrtifolia</i> and identified 15 members of the HSF family. Using a phylogenetic tree, these members were classified into three major classes and fifteen groups. The amino acid physicochemical properties of these members were also investigated. The results showed that all <i>HmHSF</i> genes are located in the nucleus, and multiple sequence alignment analysis revealed that all HmHSF proteins have the most conserved DBD structural domains. Interestingly, a special HmHSF15 protein was found in the three-dimensional structure of the protein, which has a conserved structural domain that performs a function in addition to the unique structural domain of HSF proteins, resulting in a three-dimensional structure for HmHSF15 that is different from other HmHSF proteins. GO enrichment analysis shows that most <i>HmHSFA</i>-like genes are part of various biological processes associated with abiotic stresses. Finally, this study analyzed the tissue specificity of <i>HmHSF</i> genes in different parts of <i>H. myrtifolia</i> by qRT-PCR and found that <i>HmHSF</i> genes were more abundantly expressed in roots than in other tissues, and <i>HmHSF05</i>, <i>HmHSF12</i>, and <i>HmHSF14</i> genes were different from other <i>HSF</i> genes, which could be further analyzed to verify their functionality. The results provide a basis for analyzing the functions of <i>HmHSF</i> genes in <i>H. myrtifolia</i> and help to explore the molecular regulatory mechanism of <i>HmHSF</i> in response to drought stress.